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HBOT inhibits mitochondrial dysfunction and autophagy and protects myocardial cells


Studies have shown that hyperbaric oxygen therapy (HBOT) improves heart function mainly by reducing oxygen stress, regulating energy metabolism and inhibiting cell apoptosis.

 

1. Evaluation of cardioprotective effects of HBOT

 

The cardioprotective effects of HBOT are mainly evaluated by ELISA, fluorescent probes, transmission electron microscopy and reverse transcription- quantitative PCR (RT-qPCR).

 

As observed with the transmission electron microscope, using a 0.25MPa hyperbaric chamber for HBOT for 14 days (once a day) has improved mitochondrial morphology and reduced the number of autophagic vesicles.

 

HBOT significantly increased the opening levels of ATP, ADP, energy charge and mitochondrial permeability transition pores, but decreased the levels of AMP, cytochrome c and reactive oxygen species, which has been determined using RTqPCR or immunohistochemistry. In addition, HBOT significantly increased the gene or protein expression levels of eIF4E binding protein 1, mammalian target of rapamycin (mTOR), mitochondrial DNA, NADH dehydrogenase subunit 1, mitofusin 1 and mitofusin 2, while reduced the gene or protein expression levels of autophagy-related 5 (Atg5), cytochrome c, motility-related protein 1, and p53.

 

 

2. The data show the beneficial aspects of HBOT for the treatment of MIRI

 

Data are expressed as the mean ± SD (n=5). *P<0.05; **P<0.01; ***P<0.001.

 

hbot-inhibits-mitochondrial-dysfunction.png

1. The effect of HBOT on the expression levels of ATP, ADP, AMP and cytochrome c and EC in myocardial infarction tissue.

 

(ABD) ATP, ADP, EC are determined in Sham group, IR group and HBO group.

(CE) Use ELISA kit to detect in Sham group, IR group and HBO group

 

HBO, hyperbaric oxygen; EC, energy charge; IR, ischemia/reperfusion.

2-evaluation-of-cardioprotective-effects-of-hbot.png

 

2. The effect of HBOT on the opening level of ROS and mPTP in myocardial tissue cells.

HBO, hyperbaric oxygen; ROS, reactive oxygen species; mPTP, mitochondrial permeability transition pore; IR, ischemia/reperfusion; DHE, dihydroethidium.


 

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3. The effect of HBOT on the ultrastructure of myocardium.


(A) Representative photomicrographs of myocardial tissue in the Sham group, IR group and HBO group. The mitochondria in the Sham group were normal, and no autophagic vesicles were observed. Compared with the Sham group, significant damage to mitochondria and an increase in the number of autophagic vacuoles were observed in the IR group. Compared with the IR group, only minor damage to mitochondria and a decrease in the number of autophagic vesicles (concentric circle changes) were observed in the HBO group. Yellow arrows indicate autophagic vesicles.

 

(B) Semi-quantification of the number of autophagic vesicles in Sham group, IR group and HBO group (n=5).

 

IR, ischemia/reperfusion; HBO, hyperbaric oxygen.

 

4-evaluation-of-cardioprotective-effects-of-hbot.png 

 

4. The effect of HBOT on the expression levels of 4E-BP1, Atg5 and mTOR in myocardial tissue.

 

(A) Immunohistochemical staining of myocardial sections to detect the expression levels of 4E-BP1, Atg5 and mTOR in the Sham group, IR group and HBO group. The nucleus was stained blue, and the target protein was stained brown.

 

Semi-quantification of (B) 4E-BP1, (C) Atg5 and (D) mTOR expression levels in Sham group, IR group and HBO group. Data are normalized to the Sham group and expressed as percentages.

 

IR, ischemia/reperfusion; HBO, hyperbaric oxygen; Atg5, autophagy-related 5; 4E-BP1, eukaryotic initiation factor 4E binding protein 1; mTOR, mammalian target of rapamycin.

 

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5. The effect of HBOT on the copy number of mtDNA.

 

Data are normalized to the Sham group and expressed as percentages. mtDNA, mitochondrial DNA.

6-evaluation-of-cardioprotective-effects-of-hbot.png

 

 

 

 

 

6. Statistical analysis of reverse transcription quantitative PCR results.

 

(AE) The effect of HBO pretreatment on the mRNA expression levels of mitochondrial dynamics related genes: (A) ND1, (B) cytochrome c, (C) Drp1, (D) Mfn1 and (E) Mfn2 in Sham group and IR group And the HBO group.

 

The effect of HBOT on the expression level of autophagy-related genes: (F) mTOR and (G) p53 were in the Sham group, IR and HBO group.

 

HBO, hyperbaric oxygen; IR, ischemia/reperfusion; ND1, NADH dehydrogenase subunit 1; Drp1, dynein related protein 1; Mfn1, mitofusin 1; Mfn2, mitofusin 2.

 

In summary, it has been observed that HBOT treatment protects cardiomyocytes during myocardial ischemia reperfusion injury (MIRI) by preventing mitochondrial dysfunction and inhibiting autophagy.

 

Therefore, these results provide new evidence to support the use of HBOT as a potential method of mitigating MIRI.

 

Cr: https://pubmed.ncbi.nlm.nih.gov/32901878/


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